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Evidence Guide: MSL974025 - Prepare tissue and cell cultures

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSL974025 - Prepare tissue and cell cultures

What evidence can you provide to prove your understanding of each of the following citeria?

Maintain records

  1. Maintain records of batches of media
  2. Complete records of tissue cultures so that they are retrievable, legible and accurate
  3. Complete records in accordance with information management, records, quality system and legal requirements
Maintain records of batches of media

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Complete records of tissue cultures so that they are retrievable, legible and accurate

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Complete records in accordance with information management, records, quality system and legal requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Work safely according to the legal and regulatory framework

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify hazards and workplace controls associated with the sample, preparation methods, reagents and equipment

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select, fit and use personal protective equipment (PPE)

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Address hazards and incidents as they arise

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain a chain of custody, traceable to the worker, for all cells and tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare and test cell and tissue culture media

  1. Select and confirm media specifications and processes/methods
Select and confirm media specifications and processes/methods

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare culture media to suit the application

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Sterilise culture media and check for sterility

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform quality control checks to ensure that culture media is fit for purpose

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Store culture media in accordance with specifications

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare tissue or cell cultures

  1. Select tissue/cell sample to optimise growth and prepare it for culture
Select tissue/cell sample to optimise growth and prepare it for culture

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Add specified growth agents and/or nutrients

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inoculate culture medium using aseptic techniques

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Monitor tissue or cell culture

  1. Incubate culture in specified conditions
Incubate culture in specified conditions

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Monitor growth of culture and record appearance and characteristics

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Report presence or absence of contamination

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Subculture the culture to continue the cell line

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Dispose of biohazardous and other laboratory waste safely

  1. Maintain records of batches of media
Maintain records of batches of media

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Complete records of tissue cultures so that they are retrievable, legible and accurate

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Complete records in accordance with information management, records, quality system and legal requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Required Skills and Knowledge